Stage 1 of the 2023 Giro d'Italia Donne was a flat stage that took place on .

The stage started in Chianciano Terme and finished in Chianciano Terme, starting at with an expected finish at (average speed of 43 km/h).

The total distance of the stage was 4.4 km. The stage included 0 categorized climb(s) and 0 checkpoint(s), and had a total elevation gain of 30 m, and a total descent of -61 m.